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This document specifies general requirements and guidance for metagenomics-dedicated sample preparation, and generating and analysing metagenomics sequence data obtained from massive parallel sequencing platforms. The specified metagenomics process includes the following stages:a) sampling strategy and process, including type, storage, transportation, extraction, quality;b) nucleic acid library preparationc) design and review process including sequencing strategy and assessment;d) database construction;e) bioinformatics analysis and reportf) validation and verification for bioinformatics pipeline, and databaseThis document applies to laboratories and research organizations.