Alcance
This document specifies a method for the analysis of phenolic antioxidants by high-performance liquid chromatography (HPLC).It is applicable to quantifying the following synthetic phenolic compounds added to animal and vegetable fats, oils and shortenings as antioxidants, at concentrations normally added to oils:— propyl gallate (PG);— octyl gallate (OG);— dodecyl gallate (also called “lauryl gallate (LG)”);— 2,4,5-trihydroxybutyrophenone (THBP);— tert-butylhydroquinone (TBHQ);— nordihydroguaiaretic acid (NDGA);— 2- and 3-tert-butyl-4-hydroxyanisole (BHA);— 2,6-di-tert-butyl-4-(hydroxymethyl)phenol (BHT Alcohol or Ionox-100);— 2,6-di-tert-butyl-4-hydroxytoluene (BHT).A method for determining the absence of an antioxidant, or the maximum trace amount, within the limits of the analysis, is given in Annex B.The issue of canolol, a naturally occurring substance in rapeseed, interfering with the analysis is addressed in Annex C.