ISO/TS 20224-5:2020

Molecular biomarker analysis -- Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR -- Part 5: Goat DNA detection method
Autor: ISO
Código ICS: 67.050


This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of goat-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of goat material derived from goat (Capra hircus).The target sequence is a partial fragment of the goat chromosome 9 DNA sequence (i.e. GenBank accession number NC_030816.1)[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD95 %).

Especificaciones de la Norma
Fecha de Publicación 31/07/2020
Título Secundario Analyse de biomarqueurs moléculaires -- Détection de matériaux d'origine animale dans les denrées alimentaires et les aliments pour animaux par PCR en temps réel -- Partie 5: Méthode de détection de l'ADN de chèvre
Páginas Técnicas 14
Idioma Inglés