ISO/TS 20224-6:2020

Molecular biomarker analysis -- Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR -- Part 6: Horse DNA detection method
Autor: ISO
Código ICS: 67.050


This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of horse-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of horse material derived from domestic horse (Equus caballus), mule (Equus caballus ♀ � Equus asinus ♂), hinny (Equus caballus ♂ � Equus asinus ♀) and zebroid (Equus caballusEquus simplicidens). The assay also detects the species Przewalski's horse (Equus przewalskii) and zebra (Equus burchellii).The target sequence is an Equus caballus isolate Twilight breed thoroughbred chromosome 28, EquCab3.0, whole genome shotgun sequence (i.e. GenBank accession number NC_009171.3)[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD95 %).

Especificaciones de la Norma
Fecha de Publicación 31/07/2020
Título Secundario Analyse de biomarqueurs moléculaires -- Détection de matériaux d'origine animale dans les denrées alimentaires et les aliments pour animaux par PCR en temps réel -- Partie 6: Méthode de détection de l'ADN de cheval
Páginas Técnicas 15
Idioma Inglés