ISO/TS 20224-7:2020

Molecular biomarker analysis -- Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR -- Part 7: Donkey DNA detection method
Autor: ISO
Código ICS: 67.050


This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of donkey-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of donkey material derived from donkey (Equus asinus), mule (Equus caballus ♀ � Equus asinus ♂) and hinny (Equus caballus ♂ � Equus asinus ♀). The assay also detects the species zebra (Equus burchellii).The target sequence is a partial fragment of the Equus asinus isolate Maral har breed Guanzhong donkey unplaced genomic scaffold, ASM130575v1 scaffold786, whole genome shotgun sequence (i.e. GenBank accession number NW_014638576.1)[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD95 %).

Especificaciones de la Norma
Fecha de Publicación 31/07/2020
Título Secundario Analyse de biomarqueurs moléculaires -- Détection de matériaux d'origine animale dans les denrées alimentaires et les aliments pour animaux par PCR en temps réel -- Partie 7: Méthode de détection de l'ADN d'âne
Páginas Técnicas 14
Idioma Inglés